Signalling
Part:BBa_K895003
Designed by: Frank Sargent Group: iGEM12_Dundee (2012-09-22)
Salty_TtrRS_two_component_system
This is a signalling system comprising two open reading frames of the ttrR and ttrS genes together with the promoter/operator of the ttrB gene from Salmonella LT2. Together, TtrR and TtrS form a canonical two-component system where TtrS is the membrane-bound sensor kinase and TtrR is the DNA-binding response regulator. The system senses tetrathionate outside the cell and activates transcription of the ttrB gene, which is part of a tetrathionate reducatase. This part has been designed so that expression of alternative reporter genes can be regulated by tetrathionate.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2550
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1179
Illegal AgeI site found at 311 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 28
[edit]
Categories
Parameters
None |